ANALISIS LEMAK SOXHLET PDF
PREPARASI SAMPEL Pengeringan sampel. Analisis Kadar Lemak dalam Bahan Pangan. PERALATAN SOXHLET Analisis Sifat Fisiko-Kimia Lemak atau. Berat lemak diperoleh dengan cara memisahkan lemak dengan pelarutnya. dll. dietil ksi Soxhlet Prinsip Analisis • Ekstraksi lemak dengan pelarut. Analisa lemak kasar: Metode ekstraksi soxhlet dengan pelarut organik Analisa serat kasar: Fraksi dari karbohidrat yang tidak larut dalam basa dan asam.
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For this reason, it is difficult to develop low-fat alternatives of many foods, because once the fat is removed some of the most important physical characteristics are lost.
Sohxlet, dipasang kondensor dan dialirkan pendingin melalui pompa. It does not determine phospholipids in milk, because they are located in the aqueous analsiis or at the boundary between the lipid and aqueous phases.
Thus even a lipid which consists of only triacylglycerols may contain a huge number of different chemical species. Nevertheless, over-consumption of certain lipid components can be detrimental to our health, e. This information can leemak used to: Dalam praktikum ini, kita melakukan dua tahap yang pertama menggunakan alat ektraktor soxhlet yang berfungsi untuk mengekstraksi kemiri sehingga pada tahap pertama ini akan diperoleh ektrak kemiri dan n-heksana.
The density of solid fat is higher than the density of liquid oil, and so there is an increase in density when a fat crystallizes and a decrease analisiis it melts. Initially, starch is added to the solution that contains the iodine and the solution goes a dark blue. After shaking the aqueous phase is separated from the non-polar solvent, placed in a test-tube, and heated for 20 minutes in boiling water, which produces a pink color.
Finally, many fats are prone to lipid oxidation, which leads to the formation of off-flavors and potentially harmful products. Accelerated Oxidation Tests Rather than determining the extent of lipid oxidation in a particular food, it is often more important to know its susceptibility to oxidation. Hasil ekstraksi dipindahkan ke rotary evaporator vacuum untuk diekstrak kembali berdasarkan titik didihnya.
Properties analidis Lipids in FoodsLipids are usually defined as those components that are soluble in organic solvents such as ether, hexane or chloroformbut are insoluble in water. The lipid content is determined by measuring the area under a peak in an NMR chemical shift spectra that corresponds to aoxhlet lipid fraction. After eoxhlet separated by the column, the concentration of each of the molecules is determined as they pass by a suitable detector e.
Analysis of the types of lipids present in a food usually requires that the lipid soshlet available in a fairly pure form.
In general, sample preparation should be carried out using an environment that minimizes any changes in the properties of the lipid fraction. Thus foods which are almost entirely lipids, such as olive oil, vegetable oil or lard, can usually be analyzed with little sample preparation. Analisis Lemak Metode Soxhlet Documents.
Bahan Adapaun bahan0bahan yang kami gunakan dalam percobaan ekstraksi soxhlet antara lain: The speed at which an ultrasonic wave travels through a material depends on the concentration of fat in a food.
Sulfuric acid is mixed with the milk, which digests the protein, generates heat, and breaks down the fat globule membrane that surrounds the droplets, thereby releasing the fat. As with any analytical procedure it is important not to alter the properties of the component being analyzed during the extraction process. Foods which contain high concentrations of unsaturated lipids are particularly susceptible to lipid oxidation, which can lead to theformation of undesirable off-flavors and aromas, as well as potentially toxic compounds e.
For most foods, however, more rigorous extraction methods are needed, such as the solvent or nonsolvent extraction methods described in the previous lecture.
The intensity of this pink color is directly related to the concentration of TBA-reactive substances in the original sample, and is determined by measuring its absorbance at nm using a UV-visible spectrophotometer. Dalam percobaan ini kami menggunakan sampel kemiri. Thus, a low analisjs of PV may represent either the initial or final stages of oxidation. This method is based on soxhlt absorbance of IR energy at a wavelength of 5.
The saponification number is then calculated from a knowledge of the weight of sample and the amount of KOH which reacted.
In the soxglet stages of lipid oxidation the conjugated dienes which are primary products are broken down into secondary products which do not adsorb UV-visible light strongly which leads to a decrease in absorbance. Kelima, dimasukkan beberapa batu didih dalam labu alas bulat. This lemai is then titrated with alkali KOH until a pinkish color appears.
Lipids can sometimes be extracted by simply applying pressure to a food to squeeze out the oil, e.
The sample preparation required in lipid analysis depends on the type of food being analyzed e. Thus the total lipid content determined by solvent extraction depends on the nature of the organic solvent used to carry out the extraction: Analisis Minyak Dan Lemak Documents.
Adulteration of fats and oils can be detected by measuring the type of lipids present, ssoxhlet comparing them with the profile expected for an unadulterated sample. Dalam ekstrkasi dapat digunakan berbagai macam pelarut, misalnya n-heksan dengan volume tertentu. Keempat, dimasukkan n-heksana ml dalam labu alat bulat.
The Babcock method takes about 45 minutes to carry out, and is precise to within 0. The stronger the affinity between a specific molecule and the matrix, lema, more its movement is retarded, and the slower it passes through the column. In practice, the efficiency of solvent extraction depends on the polarity of the lipids present compared to the polarity of the solvent.
Acid value The acid value is a measure of the amount of free acids present in soxulet given amount of fat. The flow of foods through pipes or the ease at which they can be packed into containers are also determined by their rheology.
Kedua, ditempatkan kedalam kertas saring.